Gefei Chen

Publikationer
Forskningsbidrag
CV
Populärvetenskap och samverkan

Utvalda publikationer

Article: NEURAL REGENERATION RESEARCH. 2024;19(11):2343-2344

Potential of molecular chaperones for treating Alzheimer's disease.

Chen G; Johansson J
Article: ADVANCED FUNCTIONAL MATERIALS. 2024

Spiders Use Structural Conversion of Globular Amyloidogenic Domains to Make Strong Silk Fibers

Qi X; Wang H; Wang K; Wang Y; Leppert A; Iashchishyn I; Zhong X; Zhou Y; Liu R; Rising A; Landreh M; Johansson J; Chen G
Article: SMALL. 2023;19(46):e2304031

Spider Silk Protein Forms Amyloid-Like Nanofibrils through a Non-Nucleation-Dependent Polymerization Mechanism.

Qi X; Wang Y; Yu H; Liu R; Leppert A; Zheng Z; Zhong X; Jin Z; Wang H; Li X; Wang X; Landreh M; A Morozova-Roche L; Johansson J; Xiong S; Iashchishyn I; Chen G
Article: COMMUNICATIONS BIOLOGY. 2023;6(1):497

Short hydrophobic loop motifs in BRICHOS domains determine chaperone activity against amorphous protein aggregation but not against amyloid formation.

Chen G; Leppert A; Poska H; Nilsson HE; Alvira CP; Zhong X; Koeck P; Jegerschöld C; Abelein A; Hebert H; Johansson J
Article: PROGRESS IN NEUROBIOLOGY. 2022;219:102366

S100A9 amyloid growth and S100A9 fibril-induced impairment of gamma oscillations in area CA3 of mouse hippocampus ex vivo is prevented by Bri2 BRICHOS.

Andrade-Talavera Y; Chen G; Pansieri J; Arroyo-García LE; Toleikis Z; Smirnovas V; Johansson J; Morozova-Roche L; Fisahn A
Article: RSC CHEMICAL BIOLOGY. 2022;3(11):1342-1358

Abilities of the BRICHOS domain to prevent neurotoxicity and fibril formation are dependent on a highly conserved Asp residue.

Chen G; Andrade-Talavera Y; Zhong X; Hassan S; Biverstål H; Poska H; Abelein A; Leppert A; Kronqvist N; Rising A; Hebert H; Koeck PJB; Fisahn A; Johansson J
Article: ACS CHEMICAL BIOLOGY. 2022;17(8):2201-2211

Amyloid Fibril Formation of Arctic Amyloid-β 1-42 Peptide is Efficiently Inhibited by the BRICHOS Domain.

Zhong X; Kumar R; Wang Y; Biverstål H; Ingeborg Jegerschöld C; J B Koeck P; Johansson J; Abelein A; Chen G
Article: ACS BIOMATERIALS SCIENCE & ENGINEERING. 2022;8(1):119-127

Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength.

Li X; Qi X; Cai Y-M; Sun Y; Wen R; Zhang R; Johansson J; Meng Q; Chen G
Article: FRONTIERS IN MOLECULAR BIOSCIENCES. 2022;9:812808

Molecular Chaperone BRICHOS Inhibits CADASIL-Mutated NOTCH3 Aggregation In Vitro.

Oliveira DV; Svensson J; Zhong X; Biverstål H; Chen G; Karlström H
Preprint: BIORXIV. 2021

Molecular chaperone ability to inhibit amyloid-derived neurotoxicity, but not amorphous protein aggregation, depends on a conserved pH-sensitive Asp residue

Chen G; Andrade-Talavera Y; Zhong X; Hassan S; Biverstal H; Poska H; Abelein A; Leppert A; Kronqvist N; Rising A; Hebert H; Koeck PJB; Fisahn A; Johansson J
Article: BMC BIOTECHNOLOGY. 2020;20(1):37

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies.

Cai H; Chen G; Yu H; Tang Y; Xiong S; Qi X
Article: COMMUNICATIONS BIOLOGY. 2020;3(1):32

Augmentation of Bri2 molecular chaperone activity against amyloid-β reduces neurotoxicity in mouse hippocampus in vitro.

Chen G; Andrade-Talavera Y; Tambaro S; Leppert A; Nilsson HE; Zhong X; Landreh M; Nilsson P; Hebert H; Biverstål H; Fisahn A; Abelein A; Johansson J
Article: NATURE COMMUNICATIONS. 2017;8(1):2081

Bri2 BRICHOS client specificity and chaperone activity are governed by assembly state.

Chen G; Abelein A; Nilsson HE; Leppert A; Andrade-Talavera Y; Tambaro S; Hemmingsson L; Roshan F; Landreh M; Biverstål H; Koeck PJB; Presto J; Hebert H; Fisahn A; Johansson J
Article: BIOPOLYMERS. 2016;105(7):385-392

Chimeric spider silk proteins mediated by intein result in artificial hybrid silks.

Lin S; Chen G; Liu X; Meng Q
Article: CHEMBIOCHEM. 2015;16(12):1720-1724

Diversified Structural Basis of a Conserved Molecular Mechanism for pH-Dependent Dimerization in Spider Silk N-Terminal Domains.

Otikovs M; Chen G; Nordling K; Landreh M; Meng Q; Jörnvall H; Kronqvist N; Rising A; Johansson J; Jaudzems K
Journal article: CHEMBIOCHEM. 2015;16(12):1824

Back Cover: Diversified Structural Basis of a Conserved Molecular Mechanism for pH‐Dependent Dimerization in Spider Silk N‐Terminal Domains (ChemBioChem 12/2015)

Otikovs M; Chen G; Nordling K; Landreh M; Meng Q; Jörnvall H; Kronqvist N; Rising A; Johansson J; Jaudzems K
Article: PLOS BIOLOGY. 2014;12(8):e1001921

Carbonic anhydrase generates CO2 and H+ that drive spider silk formation via opposite effects on the terminal domains.

Andersson M; Chen G; Otikovs M; Landreh M; Nordling K; Kronqvist N; Westermark P; Jörnvall H; Knight S; Ridderstråle Y; Holm L; Meng Q; Jaudzems K; Chesler M; Johansson J; Rising A
Article: PLOS ONE. 2012;7(12):e52293

Full-length minor ampullate spidroin gene sequence.

Chen G; Liu X; Zhang Y; Lin S; Yang Z; Johansson J; Rising A; Meng Q

Artiklar

Article: PROGRESS IN NEUROBIOLOGY. 2024;235:102585

Signal peptide peptidase-like 2b modulates the amyloidogenic pathway and exhibits an Aβ-dependent expression in Alzheimer's disease.

Maccioni R; Travisan C; Badman J; Zerial S; Wagener A; Andrade-Talavera Y; Picciau F; Grassi C; Chen G; Lemoine L; Fisahn A; Jiang R; Fluhrer R; Mentrup T; Schröder B; Nilsson P; Tambaro S
Journal article: NATURE COMMUNICATIONS. 2024;15(1):965

Identification of potential aggregation hotspots on Aβ42 fibrils blocked by the anti-amyloid chaperone-like BRICHOS domain.

Kumar R; Le Marchand T; Adam L; Bobrovs R; Chen G; Fridmanis J; Kronqvist N; Biverstål H; Jaudzems K; Johansson J; Pintacuda G; Abelein A
Journal article: JOURNAL OF FUNCTIONAL FOODS. 2024;113:106041

Eriodictyol downregulates UBA52 to promote autophagy and upregulates Nrf2/HO-1 to inhibit oxidative stress to ameliorate non-alcoholic fatty liver disease

Cai Y; Yuan L; Wang K; Liu Q; Xing H; Zhong P; Lin J; Liang Y; Chen G; Li W; Chen J; Li X
Article: NATURE COMMUNICATIONS. 2024;15(1):965

Identification of potential aggregation hotspots on Aβ42 fibrils blocked by the anti-amyloid chaperone-like BRICHOS domain

Kumar R; Le Marchand T; Adam L; Bobrovs R; Chen G; Fridmanis J; Kronqvist N; Biverstal H; Jaudzems K; Johansson J; Pintacuda G; Abelein A
Article: EXPERIMENTAL NEUROLOGY. 2023;369:114545

Low dose of levetiracetam counteracts amyloid β-induced alterations of hippocampal gamma oscillations by restoring fast-spiking interneuron activity.

Isla AG; Balleza-Tapia H; Chu F; Chen G; Johansson J; Nilsson P; Fisahn A
Article: SMALL. 2023;19(46)

Spider Silk Protein Forms Amyloid-Like Nanofibrils through a Non-Nucleation-Dependent Polymerization Mechanism (Small 46/2023)

Qi X; Wang Y; Yu H; Liu R; Leppert A; Zheng Z; Zhong X; Jin Z; Wang H; Li X; Wang X; Landreh M; Morozova-Roche LA; Johansson J; Xiong S; Iashchishyn I; Chen G
Journal article: IBRO NEUROSCIENCE REPORTS. 2023;15:s447

PRO-NEUROGENIC THERAPEUTIC APPROACHES BASED ON BRI2 MODULATION

Martins F; Chen G; Zhao H; Nilsson P; Tambaro S; Rebelo S
Article: BIOLOGY-BASEL. 2023;12(6):805

The Expression of the Endocannabinoid Receptors CB2 and GPR55 Is Highly Increased during the Progression of Alzheimer's Disease in AppNL-G-F Knock-In Mice.

Medina-Vera D; Zhao H; Bereczki E; Rosell-Valle C; Shimozawa M; Chen G; de Fonseca FR; Nilsson P; Tambaro S
Article: PNAS NEXUS. 2023;2(2):pgac303

A "grappling hook" interaction connects self-assembly and chaperone activity of Nucleophosmin 1.

Saluri M; Leppert A; Gese GV; Sahin C; Lama D; Kaldmäe M; Chen G; Elofsson A; Allison TM; Arsenian-Henriksson M; Johansson J; Lane DP; Hällberg BM; Landreh M
Article: MOLECULAR THERAPY. 2023;31(2):487-502

Intravenous treatment with a molecular chaperone designed against β-amyloid toxicity improves Alzheimer's disease pathology in mouse models.

Manchanda S; Galan-Acosta L; Abelein A; Tambaro S; Chen G; Nilsson P; Johansson J
Article: NATURE COMMUNICATIONS. 2022;13(1):4695

Spidroin N-terminal domain forms amyloid-like fibril based hydrogels and provides a protein immobilization platform.

Arndt T; Jaudzems K; Shilkova O; Francis J; Johansson M; Laity PR; Sahin C; Chatterjee U; Kronqvist N; Barajas-Ledesma E; Kumar R; Chen G; Strömberg R; Abelein A; Langton M; Landreh M; Barth A; Holland C; Johansson J; Rising A
Article: FREE RADICAL BIOLOGY AND MEDICINE. 2022;188:194-205

Resolution of inflammation is disturbed in acute ischemic stroke with diabetes mellitus and rescued by resolvin D2 treatment.

Tang X; Liu L; Miao Z; Zhang J; Cai X; Zhao B-Q; Chen G; Schultzberg M; Zhao Y; Wang X
Article: PROTEIN SCIENCE. 2022;31(8):e4378

ATP-independent molecular chaperone activity generated under reducing conditions.

Leppert A; Chen G; Lianoudaki D; Williams C; Zhong X; Gilthorpe JD; Landreh M; Johansson J
Article: ACTA NEUROPATHOLOGICA COMMUNICATIONS. 2022;10(1):96

Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Aβ amyloidosis.

Jiang R; Smailovic U; Haytural H; Tijms BM; Li H; Haret RM; Shevchenko G; Chen G; Abelein A; Gobom J; Frykman S; Sekiguchi M; Fujioka R; Watamura N; Sasaguri H; Nyström S; Hammarström P; Saido TC; Jelic V; Syvänen S; Zetterberg H; Winblad B; Bergquist J; Visser PJ; Nilsson P
Article: STRUCTURE. 2022;30(5):733-742.e7

A "spindle and thread" mechanism unblocks p53 translation by modulating N-terminal disorder.

Kaldmäe M; Vosselman T; Zhong X; Lama D; Chen G; Saluri M; Kronqvist N; Siau JW; Ng AS; Ghadessy FJ; Sabatier P; Vojtesek B; Sarr M; Sahin C; Österlund N; Ilag LL; Väänänen VA; Sedimbi S; Arsenian-Henriksson M; Zubarev RA; Nilsson L; Koeck PJB; Rising A; Abelein A; Fritz N; Johansson J; Lane DP; Landreh M
Article: NEUROBIOLOGY OF DISEASE. 2021;159:105514

Bri2 BRICHOS chaperone rescues impaired fast-spiking interneuron behavior and neuronal network dynamics in an AD mouse model in vitro.

Andrade-Talavera Y; Chen G; Kurudenkandy FR; Johansson J; Fisahn A
Article: SCIENTIFIC REPORTS. 2021;11(1):21069

AA amyloid in human food chain is a possible biohazard.

Rising A; Gherardi P; Chen G; Johansson J; Oskarsson ME; Westermark GT; Westermark P
Article: MICROBIAL CELL FACTORIES. 2021;20(1):150

Expression of the human molecular chaperone domain Bri2 BRICHOS on a gram per liter scale with an E. coli fed-batch culture.

Schmuck B; Chen G; Pelcman J; Kronqvist N; Rising A; Johansson J
Article: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. 2021;198:113996

The synthesis and characterization of Bri2 BRICHOS coated magnetic particles and their application to protein fishing: Identification of novel binding proteins.

Tigro H; Kronqvist N; Abelein A; Galan-Acosta L; Chen G; Landreh M; Lyashkov A; Aon MA; Ferrucci L; Shimmo R; Johansson J; Moaddel R
Article: TRANSLATIONAL PSYCHIATRY. 2021;11(1):212

Ablation of p75NTR signaling strengthens gamma-theta rhythm interaction and counteracts Aβ-induced degradation of neuronal dynamics in mouse hippocampus in vitro.

Andrade-Talavera Y; Balleza-Tapia H; Dolz-Gaitón P; Chen G; Johansson J; Fisahn A
Article: FRONTIERS IN CARDIOVASCULAR MEDICINE. 2021;8:650848

Echocardiographic Prognosis Relevance of Attenuated Right Heart Remodeling in Idiopathic Pulmonary Arterial Hypertension.

Zhao Q-H; Gong S-G; Jiang R; Li C; Chen G-F; Luo C-J; Qiu H-L; Liu J-M; Wang L; Zhang R
Article: JOURNAL OF PHYSIOLOGY-LONDON. 2020;598(17):3711-3725

Modulation of Kv3.1/Kv3.2 promotes gamma oscillations by rescuing Aβ-induced desynchronization of fast-spiking interneuron firing in an AD mouse model in vitro.

Andrade-Talavera Y; Arroyo-García LE; Chen G; Johansson J; Fisahn A
Article: FEBS JOURNAL. 2020;287(13):2823-2833

High intracellular stability of the spidroin N-terminal domain in spite of abundant amyloidogenic segments revealed by in-cell hydrogen/deuterium exchange mass spectrometry.

Kaldmäe M; Leppert A; Chen G; Sarr M; Sahin C; Nordling K; Kronqvist N; Gonzalvo-Ulla M; Fritz N; Abelein A; Laίn S; Biverstål H; Jörnvall H; Lane DP; Rising A; Johansson J; Landreh M
Article: SCIENTIFIC REPORTS. 2020;10(1):9817

Recombinant Bri3 BRICHOS domain is a molecular chaperone with effect against amyloid formation and non-fibrillar protein aggregation.

Poska H; Leppert A; Tigro H; Zhong X; Kaldmäe M; Nilsson HE; Hebert H; Chen G; Johansson J
Article: SCIENTIFIC REPORTS. 2020;10(1):235

High-yield Production of Amyloid-β Peptide Enabled by a Customized Spider Silk Domain.

Abelein A; Chen G; Kitoka K; Aleksis R; Oleskovs F; Sarr M; Landreh M; Pahnke J; Nordling K; Kronqvist N; Jaudzems K; Rising A; Johansson J; Biverstål H
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2019;294(8):2606-2615

Blood-brain and blood-cerebrospinal fluid passage of BRICHOS domains from two molecular chaperones in mice.

Tambaro S; Galan-Acosta L; Leppert A; Chen G; Biverstål H; Presto J; Nilsson P; Johansson J
Article: ELIFE. 2018;7:e37703

TrpV1 receptor activation rescues neuronal function and network gamma oscillations from Aβ-induced impairment in mouse hippocampus in vitro.

Balleza-Tapia H; Crux S; Andrade-Talavera Y; Dolz-Gaiton P; Papadia D; Chen G; Johansson J; Fisahn A
Article: FEBS JOURNAL. 2018;285(10):1873-1885

A spidroin-derived solubility tag enables controlled aggregation of a designed amyloid protein.

Sarr M; Kronqvist N; Chen G; Aleksis R; Purhonen P; Hebert H; Jaudzems K; Rising A; Johansson J
Article: CHINESE JOURNAL OF BIOTECHNOLOGY. 2016;32(12):1704-1714

[Construction of spider silk functional platform via intein trans-splicing].

Lin S; Chen G; Meng Q
Article: BIOCHEMICAL JOURNAL. 2016;473(20):3683-3704

Dementia-related Bri2 BRICHOS is a versatile molecular chaperone that efficiently inhibits Aβ42 toxicity in Drosophila.

Poska H; Haslbeck M; Kurudenkandy FR; Hermansson E; Chen G; Kostallas G; Abelein A; Biverstål H; Crux S; Fisahn A; Presto J; Johansson J
Article: CHINESE JOURNAL OF BIOTECHNOLOGY. 2014;30(8):1308-1317

[Effect of salt on minor ampullate silk spidroin modules].

Wang J; Chen G; Meng Q
Article: NATURE COMMUNICATIONS. 2014;5:3254

Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation.

Kronqvist N; Otikovs M; Chmyrov V; Chen G; Andersson M; Nordling K; Landreh M; Sarr M; Jörnvall H; Wennmalm S; Widengren J; Meng Q; Rising A; Otzen D; Knight SD; Jaudzems K; Johansson J
Article: CHINESE JOURNAL OF BIOTECHNOLOGY. 2013;29(9):1323-1331

[Expression of Araneus ventricosus minor ampullate spidroin].

Yang Z; Chen G; Meng Q
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Letter: NANO LETTERS. 2023;23(12):5836-5841

Liquid-Liquid Phase Separation Primes Spider Silk Proteins for Fiber Formation via a Conditional Sticker Domain.

Leppert A; Chen G; Lama D; Sahin C; Railaite V; Shilkova O; Arndt T; Marklund EG; Lane DP; Rising A; Landreh M
Review: PROTEIN SCIENCE. 2023;32(6):e4645

A new kid in the folding funnel: Molecular chaperone activities of the BRICHOS domain.

Leppert A; Poska H; Landreh M; Abelein A; Chen G; Johansson J
Conference publication: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 2023;52(SUPPL 1):S54

Structural insights into aggregation hotspots on Alzheimer's associated amyloid-β fibrils blocked by the BRICHOS chaperone

Abelein A; Kumar R; Le Marchand T; Adam L; Chen G; Fridmanis J; Kronqvist N; Biverstal H; Jaudzems K; Johansson J; Pintacuda G
Preprint: BIORXIV. 2022

Signal Peptide Peptidase-Like 2b affects APP cleavage and exhibits a biphasic Aβ-mediated expression in Alzheimer’s disease

Maccioni R; Travisan C; Zerial S; Wagener A; Andrade-Talavera Y; Picciau F; Grassi C; Chen G; Lemoine L; Fisahn A; Jiang R; Fluhrer R; Mentrup T; Schröder B; Nilsson P; Tambaro S
Preprint: BIORXIV. 2022

A “grappling hook” interaction balances self-assembly and chaperone activity of Nucleophosmin 1

Saluri M; Leppert A; Gese GV; Sahin C; Lama D; Kaldmäe M; Chen G; Elofsson A; Allison T; Arsenian-Henriksson M; Johansson J; Lane D; Hällberg M; Landreh M
Preprint: RESEARCH SQUARE. 2021

Resolution of Inflammation is Disturbed in Acute Ischemic Stroke With Diabetes Mellitus and Rescued By Resolvin D2 Treatment

Tang X; Liu L; Miao Z; Zhang J; Cai X; Zhao B-Q; Chen G; Schultzberg M; Zhao Y; Wang X
Preprint: BIORXIV. 2021

A “spindle and thread”-mechanism unblocks translation of N-terminally disordered proteins

Kaldmäe M; Vosselman T; Zhong X; Lama D; Chen G; Saluri M; Kronqvist N; Siau JW; Ng AS; Ghadessy F; Sabatier P; Vojtesek B; Sarr M; Sahin C; Österlund N; Ilag L; Väänänen V; Sedimbi S; Zubarev R; Nilsson L; Koeck P; Rising A; Fritz N; Johansson J; Lane D; Landreh M
Preprint: SSRN ELECTRONIC JOURNAL. 2021

A 'Spindle and Thread'-Mechanism Unblocks p53 Translation by Modulating N-Terminal Disorder

Kaldmäe M; Vosselman T; Zhong X; Lama D; Chen G; Saluri M; Kronqvist N; Sau JW; Ng AS; Ghadessy F; Sabatier P; Vojtesek B; Sarr M; Sahin C; Österlund N; Ilag L; Väänänen V; Sedimbi S; Arsenain-Henriksson M; Zubarev R; Nilsson L; Koeck P; Rising A; Abelein A; Fritz N; johansson J; Lane DP; Landreh M
Preprint: RESEARCH SQUARE. 2020

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

Cai H; Chen G; Yu H; Tang Y; Xiong S; Qi X
Preprint: RESEARCH SQUARE. 2020

One-Step Heating Strategy for Efficient Solubilization of Recombinant Spider Silk Protein From Inclusion Bodies

Cai H; Chen G; Yu H; Tang Y; Xiong S; Qi X
Preprint: RESEARCH SQUARE. 2019

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

Cai H; Chen G; Tang Y; Yu H; Xiong S; Qi X
Meeting abstract: AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS. 2019;26(sup1):152-153

BRICHOS: a chaperone with different activities depending on quaternary structure and cellular location?

Leppert A; Chen G; Johansson J
Conference publication: PROTEIN SCIENCE. 2017;26:18-19

Exploring Amyloid-like Aggregation Using a Mutant Domain of a Spidroin as a Solubility Tag

Sarr M; Kronqvist N; Reifenrath M; Chen G; Rising A; Johansson J
Conference publication: PROTEIN SCIENCE. 2017;26:31-32

Bri2 BRICHOS molecular chaperone activity is decoupled from its ability to inhibit amyloid fibril formation

Chen G; Abelein A; Leppert A; Tambaro S; Biverstal H; Presto J; Johansson J

Forskningsbidrag

BRI2 role in neuronal differentiation and the underlying molecular mechanisms: towards an innovative regenerative therapy for Alzheimer’s Disease

Fundação para a Ciência e Tecnologia

1 January 2022 - 31 December 2024

Anställningar

Senior Forskare, Medicin, Huddinge, Karolinska Institutet, 2024-
Senior Forskare, Biovetenskaper och näringslära, Karolinska Institutet, 2023-2024

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