Nutsa Burduli

Nutsa Burduli

Phd Student
Visiting address: Alfred Nobels allé 8, plan 8, 14152 Huddinge
Postal address: H7 Medicin, Huddinge, H7 Hematologi Alici, 171 77 Stockholm
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About me

  • BSc (Hons) in Biochemistry from University of Galway, Ireland (2011-2015).
    Degree project title: Mutation of a key enzyme residue in MSH3 subunit of MutSβ to inactivate its enzymatic function
    Principal investigator: Prof. Robert Lahue

    MSc in Cancer research from University of Galway, Ireland (2018-2019)
    Degree project title: Development of genetically engineered Natural Killer cells for the treatment of Pancreatic Cancer
    Principal investigator: Prof. Michael O'Dwyer
    Immediate supervisor: Dr. Subhashis Sarkar

    Currently pursuing a PhD by investigating the genetic landscape behind natural killer cell cytokine and cytotoxic responses, comparative assessment of various genetic modification strategies for NK cells and overcoming their resistance to genetic modification.
    Primary investigator: Dr. Evren Alici
    Co-supervisors: Assistant Prof. Arnika Kathleen Wagner, Dr. Stephan Meinke, Prof. Hans-Gustaf Ljunggren


    Laboratory skills:
    Multicolour flow cytometry panel design
    Multicolour flow cytometry
    Cell culture (primary and cell line)
    Cloning
    Lentiviral production
    Lentiviral transduction
    PBMC isolation and culture
    PCR
    Molecular biology
    CRISPR/Cas9-mediated gene knockouts
    Nucleofection/electroporation
    Cytotoxicity assays
    Fluorescence assisted cell sorting (FACS)
    Western blotting
    Gene insert design
    DNA/RNA isolation and purification

Research

  • NK cells
    Genetic modification
    Lymphocyte proliferation
    CRISPR/Cas9 strategies
    Lymphocyte biology
    Immunotherapies
    Cellular immunotherapies

Teaching

  • Previous student: Fernando Herrero Quevedo
    Project title: Targeted knockout of amino acid transporters ASCT1 and ASCT2 to abrogate NK cell transduction with baboon envelope pseudotyped lentiviral vectors

    Current student: Kjell Klarenbeek
    Project title: Evaluation of candidate genes regulating natural killer cell cytotoxic and cytokine responses

Articles

All other publications

Employments

  • Phd Student, Department of Medicine, Huddinge, Karolinska Institutet, 2024-2024
  • Phd Student, Department of Medicine, Huddinge, Karolinska Institutet, 2024-2024

Degrees and Education

  • Master of Science, Cancer research, Development of genetically engineered Natural Killer cells for the treatment of Pancreatic Cancer, Medicine, National University of Ireland, Galway, 2019
  • Bachelor of Science, Biochemistry, Mutation of a key enzyme residue in MSH3 subunit of MutSβ to inactivate its enzymatic function, Biological and Chemical Sciences, National University of Ireland, Galway, 2015

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